Live-Attenuated VEEV Vaccine Delivered by iDNA Using Microneedles Is Immunogenic in Rabbits
نویسندگان
چکیده
Effective and simple delivery of DNA vaccines remains a key to successful clinical applications. Previously, we developed novel class vaccines, sometimes called iDNA, which encodes the whole live-attenuated vaccine viruses. Compared standard vaccine, an iDNA required low dose launch in vitro or vivo . The goal this pilot study was investigate if encoding Venezuelan equine encephalitis virus (VEEV) can be efficiently delivered by microneedle device using single-dose vaccination with naked plasmid. For purpose, used pMG4020 plasmid V4020 VEE virus. contains structural gene rearrangement, as well attenuating mutations genetically engineered prevent reversion mutations. administered experimental rabbits hollow microstructured transdermal system (hMTS) device. No adverse events were noted. Animals that received have successfully seroconverted, high plaque reduction neutralization test (PRNT) antibody titers, similar those observed animals place We conclude
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ژورنال
عنوان ژورنال: Frontiers in tropical diseases
سال: 2022
ISSN: ['2673-7515']
DOI: https://doi.org/10.3389/fitd.2022.813671